| Type | Specification |
|---|---|
| GLP features | Early maintenance feedback (EMF), electronic records of maintenance and errors |
| Communications | Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output |
| Safety features | Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display |
| Injection range | 0.1 – 100 μl in 0.1 μl increments Up to 1500 μl with multiple draw (hardware modification required) |
| Replicate injections | 1 – 99 from one vial |
| Precision | Typically < 0.5 % RSD of peak areas from 5 – 100 μl, Typically < 1 % RSD of peak areas from 1 – 5 μl |
| Minimum sample volume | 1 μl from 5 μl sample in 100 μl microvial, or 1 μl from 10 μl sample in 300 μl microvial |
| Carryover | Typically < 0.1 %, < 0.05 % with external needle cleaning |
| Sample viscosity | range 0.2 – 50 cp |
| Replicate injections per vial | 1 – 99 |
| Sample capacity |
100 × 2-ml vials in 1 tray 40 × 2-ml vials in ½ tray 15 × 6-ml vials in ½ tray (Agilent vials only) |
| Injection cycle time | Typically 50 s depending on draw speed and injection volume |
| Type | Specification |
|---|---|
Hydraulic system |
Dual plunger in series pump with proprietary servo-controlled variable stroke drive, floating plungers and active inlet valve |
Setable flow range |
0.001 – 10 ml/min, in 0.001 ml/min increments |
Flow range |
0.2 – 10.0 ml/min |
Flow precision |
< 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min |
Pressure |
Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5880 psi) up to 5 ml/min Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 ml/min |
Pressure pulsation |
< 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, |
Compressibility compensation |
User-selectable, based on mobile phase compressibility |
Recommended pH |
1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack |
Range |
stainless steel |
Gradient formation |
Low pressure quaternary mixing/gradient capability using proprietary |
Composition Range |
0 – 95 % or 5 – 100 %, user selectable |
Composition Precision |
< 0.2 % RSD, at 0.2 and 1 ml/min |
Control and data evaluation |
Agilent ChemStation for LC |
Analog output |
For pressure monitoring, 2 mV/bar, one output |
Communications |
Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional |
| Type | Specification |
|---|---|
Temperature range |
10 degrees below ambient to 80 °C |
Temperature stability |
± 0.15 °C |
Temperature accuracy |
± 0.8 °C |
Column capacity |
Three 30 cm |
Warm-up/cool-down |
5 minutes from ambient to 40 °C |
Time |
10 minutes from 40 – 20 °C |
Dead volume |
3 μl left heat exchanger |
Dimensions |
6 μl right heat exchanger |
(h × w × d) |
(5.5 × 16 × 17 inches) |
Weight |
10.2 kg (22.5 lbs) |
Communications |
Controller-area network (CAN), GPIB, |
| Type | Specification |
|---|---|
Detection type |
Double-beam photometer |
Light source |
Deuterium lamp |
Wavelength range |
190–600 nm |
Short term noise |
± 0.75 × 10-5 AU at 254 nm |
(ASTM) |
|
Drift |
3 × 10-4 AU/hr at 254 |
Linearity |
> 2 AU (5%) upper limit |
Wavelength accuracy |
± 1 nm Self-calibration with deuterium lines, |
|
verification with holmium oxide filter |
Band width |
6.5 nm typical |
Flow cells |
Standard: 14-μl volume, 10-mm |
|
cell path length and 40 bar |
|
(588 psi) pressure maximum |
|
High pressure: 14-μl volume, |
|
10-mm cell path length and |
|
400 bar (5880 psi) pressure |
|
maximum |
|
Micro: 1-μl volume, 5-mm cell |
|
path length and 40 bar (588 psi) |
|
pressure maximum |
|
Semimicro: 5-μl volume, 6-mm |
|
cell path length and 40 bar |
|
(588 psi) pressure maximum |
Control and data |
Agilent ChemStation for LC |
evaluation |
|
Analog outputs |
Recorder/integrator: 100 mV or |
|
1 V, output range 0.001 – 2 AU, |
|
one output |
Communications |
Controller-area network (CAN), |
|
GPIB, RS-232C, APG Remote: |
|
ready, start, stop and |
|
shut-down signals, LAN |
|
optional |
| Type | Specification |
|---|---|
Detection type |
1024-element photodiode array |
Light source |
Deuterium and tungsten lamps |
Wavelength range |
190 – 950 nm |
Short term noise (ASTM) |
± 1 × 10-5 AU at 254 and 750 nm |
|
|
Single and Multi-Wavelength Drift |
2 × 10-3 AU/hr at 254 nm |
|
|
|
|
Linear absorbance range |
> 2 AU (upper limit) |
|
|
Wavelength accuracy |
± 1 nm Self-calibration with deuterium lines, |
|
verification with holmium oxide filter |
Wavelength bunching |
1 – 400 nm Programmable in steps of 1 nm |
Slit width |
1, 2, 4 , 8, 16 nm Programmable slit |
Diode width |
< 1 nm |
Flow cells |
Standard: 13 μl volume, 10 mm cell path |
|
length and 120 bar (1760 psi) |
|
pressure maximum |
|
Semi-Micro: 5 μl volume, 6 mm cell |
|
path length and 120 bar (1760 psi) |
|
pressure maximum |
|
High pressure: 1.7 μl volume, 6 mm cell |
|
path length and 400 bar (5880 psi) |
|
pressure maximum |
|
500 nano: 0.5 μl volume, 10 mm cell |
|
path length and 50 bar (725 psi) |
|
pressure maximum |
Control and data evaluation |
Agilent ChemStation for LC |
Analog outputs |
Recorder/integrator: 100 mV or 1 V, |
|
output range 0.001 – 2 AU, two outputs |
Communications |
Controller-area network (CAN), GPIB, |
|
RS-232C, APG Remote: ready, start, |
|
stop and shut-down signals, LAN optional |